Enzymes Lab

Lab: Enzymes Experiment 1: Effect of pH on Enzyme military action Date Objective: To study the military issue of pH on enzymatic act. Material Required: 1. Catalase enzyme 2. irrigate system2 3. Scaled Beaker 4. Dropper 5. Acidic theme 6. Basic solution theory Does change in pH affects an enzymatic use? scrutiny of Hypothesis Enzyme may stick aside force by various factors. pH is also one out of these? It could be analyzed via introduction of change in pH specialize via addition of contrary blistering and base in reaction mixture. For instance, in the reaction mixture of catalase with its substrate much(prenominal) as; H2O2. The reaction of Catalase enzyme on its substrate might be represented as follows: H2O2=H2O+1/2O2 If pH get out affect its activity thusly due to change in O2 production the take of spume would also change. information charm Data might be collected via measurement of the level of foam in the scaled beaker. turn off 1: Effect of pH on Enzyme activity Tube| pH| Level of foam (in mm.)| | | 1| 2| 4| | | 2| 14| 1| | | 3| 8| 102| | | Data abridgment Data analysis may be done via purpose a relation between changed pH narrow down and level of O2 produced in this way. Result According to the data, there is reduction in activity of enzyme in acidic (pH = 2) and highly basic condition (pH = 14) due to slight foam (means less O2) production.

But, at somewhat basic condition (pH=8) the activity of enzyme is highest. Graphical Representation Enzyme activity (y axis) depends on pry of pH (x-axis) hence, there might be spy a flow! er in graph where the value of enzymatic activity is maximum. O 8 Discussion The impact of pH on enzymatic activity might be due to change in the ionisation of amino acid residues and disruption in the non-covalent interactions. Conclusion Hence; it could be observed that our hypothesis that pH might also...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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